import arcpy
from arcpy import mapping
fclayer = "fclayer"arcpy.MakeFeatureLayer_management(source, fclayer)layer = mapping.Layer(fclayer)layer.definitionQuery = "UIDField = 'unique'"uRows = arcpy.UpdateCursor(layer)row = Nonefor row in uRows: row.URL = url uRows.updateRow(row)del rowdel uRowsdel layerdel fclayerFor more information check out the help page here.
Enjoy